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RBD-Rabbit Fc

R3,591.00 – R8,012.81

We have produced this RBD protein recombinantly in our plants and fused the gene to a Rabbit IgG Fc peptide tag.

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SKU: Ag_101 Categories: , Tag:

Description

The receptor binding domain, locating within the S1 subunit of the Spike Protein of the new coronavirus is how the virus enters host cells, using the host ACE-2 receptor. We have produced this RBD protein recombinantly in our plants and fused the gene to a Rabbit IgG Fc peptide tag. This allows easy binding to protein A and detection using common anti-Rabbit IgG-HRP secondary antibodies. This protein is available in 100ug research amounts, as well as bulk mg amounts. It’s a preferred protein for two of our local test kit manufacturers and has been proven to bind the ACE-2 receptor in ELISA and SPR studies.

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OVERVIEW

Product Name SARS-CoV-2 Spike RBD, Rabbit IgG Fc Tag
Source SARS-CoV-2 truncated S1 protein from SARS-CoV-2 (aa 281-698) (Genbank # MN908947.3) fused to the fragment crystallizable region of Rabbit IgG1 (Genbank # L29172.1).
Expression host Nicotiana benthamiana
Tag None
Description The receptor binding domain (RBD) of SARS-CoV-2 fused to a Rabbit IgG1 fragment crystallizable (Fc) region was recombinantly expressed in Nicotiana benthamiana plants.
Concentration 2.0 mg/ml +-20%, or variable
Volume Variable
Storage Short term (up to 1 week): 2 – 8 C
Long term: -20 C (Aliquot and avoid freeze thaw cycles)

PROPERTIES

Form Liquid
Colour Clear to light yellow liquid
Preparation instructions None. Store immediately.
Storage buffer 0.1M Phosphate Buffered saline, pH 7.4
Preservative: none
Purification notes This product was purified by Protein A affinity chromatography.
Purity ≥75% as determined by SDS-PAGE
Stability Stability studies are in progress. Stability data to date for similar products demonstrates stability for 9 months from manufacturing date when frozen (-20 C).
General notes The spike glycoprotein of SARS-CoV-2 is known to interact with the ACE2 receptor on human respiratory epithelial cells. The receptor binding domain (RBD) is part of the S1 subunit of the spike and is responsible for recognising the ACE2 receptor.

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